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OU Actinobacillus actinomycetemcomitans Sequence Blast Server Results

TBLASTN 1.3.9 [29-Oct-93]

Reference:  Altschul, Stephen F., Warren Gish, Webb Miller, Eugene W. Myers,

and David J. Lipman (1990).  Basic local alignment search tool.  J. Mol. Biol.

215:403-410.

Notice:  statistical significance is estimated under the assumption that the

equivalent of one complete reading frame of the database codes for protein and

that significant alignments will involve only coding reading frames.

Query=  GI|126355|SP|P16462|HLYA_ACTAC LEUKOTOXIN

        (1050 letters)

Database:  /act/abi/actphrap/auto_act

           396 sequences; 1,971,947 total letters.

Searching..................................................done

                                                                     Smallest

                                                                     Poisson

                                                     Reading  High  Probability

Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N

Contig634                                                 -2  4713  0.0       1

Contig494                                                 +3    53  0.54      2

Contig393                                                 +1    45  0.70      5

Contig623                                                 -2    59  0.78      1

Contig611                                                 +1    45  0.94      4

Contig612                                                 +1    55  0.96      2

Contig577                                                 +3    56  0.98      1

Contig368                                                 -3    55  0.993     1

>Contig634

          Length = 25,694

  Minus Strand HSPs:

 Score = 4713 (2141.2 bits), Expect = 0.0, P = 0.0

 Identities = 930/990 (93%), Positives = 943/990 (95%), Frame = -2

Query:     1 MATTTLPNTKQQAAQFANSVADRAKENIDAAKEQLQKALDKLGKTGKKLTLYIPKNYKKG 60

             MATTTLPNTKQQAAQFANSVADRAKENIDAAKEQLQKALDKLGKTGKKLTLYIPKNYKKG

Sbjct:  7678 MATTTLPNTKQQAAQFANSVADRAKENIDAAKEQLQKALDKLGKTGKKLTLYIPKNYKKG 7499

Query:    61 NGLTALIKAAQKLGIEVYHEGKDGPALTNGILNTGKKLLGLTERGLTLFAPELDKWIQGN 120

             NGLTALIKAAQKLGIEVYHEGKDGPALTNGILNTGKKLLGLTERGLTLFAPELDKWIQGN

Sbjct:  7498 NGLTALIKAAQKLGIEVYHEGKDGPALTNGILNTGKKLLGLTERGLTLFAPELDKWIQGN 7319

Query:   121 KHLSNSVGSTGNLTKAIDKVQSVLGTLQAFLNTAFSGMDLDALIKARQNGKNVTDVQLAK 180

             KHLSNSVGSTGNLTKAIDKVQSVLGTLQAFLNTAFSGMDLDALIKARQNGKNVTDVQLAK

Sbjct:  7318 KHLSNSVGSTGNLTKAIDKVQSVLGTLQAFLNTAFSGMDLDALIKARQNGKNVTDVQLAK 7139

Query:   181 ASLNLINELIGTISSITNNVDTFSKQLNKLGEALGQVKHFGSFGDKLKNLPKLGNLGKGY 240

             ASLNLINELIGTISSITNNVDTFSKQLNKLGEALGQVKHFGSFGDKLKNLPKLGNLGKG 

Sbjct:  7138 ASLNLINELIGTISSITNNVDTFSKQLNKLGEALGQVKHFGSFGDKLKNLPKLGNLGKGL 6959

Query:   241 GALSGVLSAISAALLLANKHADTATKAAAAAELTNKVLGNIGKAITQYLIAQRAAAGLST 300

             GALSGVLSAISAALLLANK ADTATKAAAAAELTNKVLGNIGKAITQYLIAQRAAAGLST

Sbjct:  6958 GALSGVLSAISAALLLANKDADTATKAAAAAELTNKVLGNIGKAITQYLIAQRAAAGLST 6779

Query:   301 TGPVAGLIASVVSLAISPLSFLGIAKQFDRARMLEAYSKRFKKFGYNGDSLLGQFYKNTG 360

             TGPVAGLIASVVSLAISPLSFLGIAKQFDRARMLE YSKRFKKFGYNGDSLLGQFYKNTG

Sbjct:  6778 TGPVAGLIASVVSLAISPLSFLGIAKQFDRARMLEEYSKRFKKFGYNGDSLLGQFYKNTG 6599

Query:   361 IADAAITTINTVLSAIAAGVGAASAGSLVGAPIGLLVSAITSLISGILDASKQAVSEHIA 420

             IADAAITTINTVLSAIAAGVGAASAGSLVGAPIGLLVSAITSLISGILDASKQAV EHIA

Sbjct:  6598 IADAAITTINTVLSAIAAGVGAASAGSLVGAPIGLLVSAITSLISGILDASKQAVFEHIA 6419

Query:   421 NQLADKIKAWENKYGKNYSENGYDARHSAFLEDSLKLFNELREKYKTENILSITQQGWDQ 480

             NQLADKIKAWENKYGKNY ENGYDARHSAFLEDSLKLFNELREKYKTENILSITQQGWDQ

Sbjct:  6418 NQLADKIKAWENKYGKNYFENGYDARHSAFLEDSLKLFNELREKYKTENILSITQQGWDQ 6239

Query:   481 RIGELAGITRNGDRIQSGKAYVDYLKKGEELAKHSDKFTKQILDPIKGNIDLSGIKGSTT 540

             RIGELAGITRNGDRIQSGKAYVDYLKKGEELAKHSDKFTKQILDPIKGNIDLSGIKGSTT

Sbjct:  6238 RIGELAGITRNGDRIQSGKAYVDYLKKGEELAKHSDKFTKQILDPIKGNIDLSGIKGSTT 6059

Query:   541 LTFLNPLLTAGKEERKTRQSGKYEFITELKVKGRTDWKVKGVPNSNGVYDFSNLIQHAVT 600

             LTFLNPLLTAGKEERKTRQSGKYEFITELKVKGRTDWKVKGVPNSNGVYDFSNLIQHAVT

Sbjct:  6058 LTFLNPLLTAGKEERKTRQSGKYEFITELKVKGRTDWKVKGVPNSNGVYDFSNLIQHAVT 5879

Query:   601 RDNKVLEARLIANLGAKDDYVFVGSGSTIVNAGDGYDVVDYSKGRTGALTIDGRNATKAG 660

             RDNKVLEARLIANLGAKDDYVFVGSGSTIVNAGDGYDVVDYSKGRTGALTIDGRNATKAG

Sbjct:  5878 RDNKVLEARLIANLGAKDDYVFVGSGSTIVNAGDGYDVVDYSKGRTGALTIDGRNATKAG 5699

Query:   661 QYKVERDLSGTQVLQETVSKQETKRGKVTDLLEYRNYKLDYYYTNKGFKAHDELNSVEEI 720

             QYKVERDLSGTQVLQETVSKQETKRGKVTDLLEYRNYKLDYYYTNKGFKAHDELNSVEEI

Sbjct:  5698 QYKVERDLSGTQVLQETVSKQETKRGKVTDLLEYRNYKLDYYYTNKGFKAHDELNSVEEI 5519

Query:   721 IGSNLRDKFYGSKFNDVFHGHDGDDLIYGYDGDDRLYGDNGNDEIHGGQGNDKLYGGAGN 780

             IGS LRDKFYGSKFNDVFHGHDGDDLIYGYDGDDRLYGDNGNDEIHGGQGNDKLYGGAGN

Sbjct:  5518 IGSTLRDKFYGSKFNDVFHGHDGDDLIYGYDGDDRLYGDNGNDEIHGGQGNDKLYGGAGN 5339

Query:   781 DRLFGEYGNNYLDGGEGDDHLEGGNGSDILRGGSGNDKLFGNQGDDLLDGGEGDDQLAGG 840

             DRLFGEYGNNYLDGGEGDDHLEGGNGSDILRGGSGNDKLFGNQGDDLLDGGEGDDQLAGG

Sbjct:  5338 DRLFGEYGNNYLDGGEGDDHLEGGNGSDILRGGSGNDKLFGNQGDDLLDGGEGDDQLAGG 5159

Query:   841 EGNDIYVYRKEYGHHTITEHSGDKDKLSLANINLKDVSFERNGNDLLLKTNNRTAVTFKG 900

             EGNDIYVYRKEYGHHTITEHSGDKDKLSLANINLKDVSFERNGNDLLLKTNNRTAVTFKG

Sbjct:  5158 EGNDIYVYRKEYGHHTITEHSGDKDKLSLANINLKDVSFERNGNDLLLKTNNRTAVTFKG 4979

Query:   901 WFSKPNSSAGLDEYQRKLLEYAPEKDVHDLRDNLSYSEVKSTNHSIIKLKKLSVKMGSGL 960

             WFSKPNSSAGLDEYQRKLLEYAPEKD   L+        K       K++++  K G  +

Sbjct:  4978 WFSKPNSSAGLDEYQRKLLEYAPEKDRARLKRQFELQRGKVDKSLNNKVEEIIGKDGERI 4799

Query:   961 LRKTLIIFLIRVGTKRQFHLKSLPDLLRIK 990

               + +     + G K+    + L  L++ K

Sbjct:  4798 TSQDIDNLFDKSGNKKTISPQELAGLIKNK 4709

 Score = 623 (283.0 bits), Expect = 1.0e-157, Poisson P(2) = 1.0e-157

 Identities = 124/124 (100%), Positives = 124/124 (100%), Frame = -3

Query:   927 VHDLRDNLSYSEVKSTNHSIIKLKKLSVKMGSGLLRKTLIIFLIRVGTKRQFHLKSLPDL 986

             VHDLRDNLSYSEVKSTNHSIIKLKKLSVKMGSGLLRKTLIIFLIRVGTKRQFHLKSLPDL

Sbjct:  4899 VHDLRDNLSYSEVKSTNHSIIKLKKLSVKMGSGLLRKTLIIFLIRVGTKRQFHLKSLPDL 4720

Query:   987 LRIKVSQVALCLLLVRQVCLHKSPVCQMILVVLFQQPVVLVHPVKRYPLRHCRPIITLTL 1046

             LRIKVSQVALCLLLVRQVCLHKSPVCQMILVVLFQQPVVLVHPVKRYPLRHCRPIITLTL

Sbjct:  4719 LRIKVSQVALCLLLVRQVCLHKSPVCQMILVVLFQQPVVLVHPVKRYPLRHCRPIITLTL 4540

Query:  1047 TQIR 1050

             TQIR

Sbjct:  4539 TQIR 4528

>Contig494

          Length = 5164

  Plus Strand HSPs:

 Score = 53 (24.1 bits), Expect = 9.8, P = 1.0

 Identities = 14/48 (29%), Positives = 25/48 (52%), Frame = +3

Query:   363 DAAITTINTVLSAIAAGVGAASAGSLVGAPIGLLVSAITSLISGILDA 410

             D ++ +I  +  A AA +  A    L+  P+ LL+  +   ISG++ A

Sbjct:  2853 DLSVGSILALTGAFAASMVGAELPVLLVIPLVLLIGTLLGGISGVIVA 2996

 Score = 49 (22.3 bits), Expect = 0.78, Poisson P(2) = 0.54

 Identities = 12/30 (40%), Positives = 18/30 (60%), Frame = +2

Query:   412 KQAVSEHIANQLADKIKAWENKYGKNYSEN 441

             KQAV+EH  + LA++   ++   G N  EN

Sbjct:  4133 KQAVAEHKFDVLANQPADFDRTKGLNVMEN 4222

>Contig393

          Length = 2548

  Plus Strand HSPs:

 Score = 44 (20.0 bits), Expect = 8.5, Poisson P(2) = 1.0

 Identities = 8/33 (24%), Positives = 20/33 (60%), Frame = +1

Query:    12 QAAQFANSVADRAKENIDAAKEQLQKALDKLGK 44

             QA++  ++  + AK+  DAA +++ +  D + +

Sbjct:   343 QASEMKDAAVEAAKDAKDAAADKMAEVKDAISE 441

 Score = 44 (20.0 bits), Expect = 8.5, Poisson P(2) = 1.0

 Identities = 12/40 (30%), Positives = 20/40 (50%), Frame = +1

Query:     1 MATTTLPNTKQQAAQFANSVADRAKENIDAAKEQLQKALD 40

             MA   +   K   ++  +++A +A E  DAA E  + A D

Sbjct:   277 MAADKMAEVKDVVSEKMDAMATQASEMKDAAVEAAKDAKD 396

 Score = 39 (17.7 bits), Expect = 20., Poisson P(3) = 1.0

 Identities = 7/20 (35%), Positives = 11/20 (55%), Frame = +3

Query:    98 LLGLTERGLTLFAPELDKWI 117

             L  + E+G  L  P+ D W+

Sbjct:  1581 LQNIREKGFYLQTPKKDDWL 1640

 Score = 38 (17.3 bits), Expect = 4.5, Poisson P(4) = 0.99

 Identities = 9/23 (39%), Positives = 14/23 (60%), Frame = +3

Query:    33 EQLQKALDKLGKTGKKLTLYIPK 55

             +Q++K    L KTG+KL    P+

Sbjct:   624 KQIKKQSKLLFKTGQKLIYPFPQ 692

 Score = 37 (16.8 bits), Expect = 1.2, Poisson P(5) = 0.70

 Identities = 8/27 (29%), Positives = 14/27 (51%), Frame = +2

Query:   207 LNKLGEALGQVKHFGSFGDKLKNLPKL 233

             L+ +GE   +V+H     + +  L KL

Sbjct:  1706 LSSIGETKPKVRHHTVVKESISRLAKL 1786

>Contig623

          Length = 18,417

  Minus Strand HSPs:

 Score = 59 (26.8 bits), Expect = 1.5, P = 0.78

 Identities = 15/80 (18%), Positives = 37/80 (46%), Frame = -2

Query:   937 SEVKSTNHSIIKLKKLSVKMGSGLLRKTLIIFLIRVGTKRQFHLKSLPDLLRIKVSQVAL 996

             S+   + H+ +  +     +   L+ +   ++ + V T+R FH    P  L   VS+V++

Sbjct:  5765 SQFDFSKHNALTNQAFRRNIARDLVTRGHTLWTVTVDTERSFHCNYRPSHLSAIVSRVSI 5586

Query:   997 CLLLVRQVCLHKSPVCQMIL 1016

               L + +    ++ +C+  +

Sbjct:  5585 FYLSLFRHLFRQTKICKRFI 5526

>Contig611

          Length = 11,498

  Plus Strand HSPs:

 Score = 44 (20.0 bits), Expect = 1.4e+02, P = 1.0

 Identities = 9/21 (42%), Positives = 10/21 (47%), Frame = +1

Query:    75 IEVYHEGKDGPALTNGILNTG 95

             +  Y E KDG  L  GI   G

Sbjct:  6979 VAYYREAKDGQVLQEGINELG 7041

 Score = 44 (20.0 bits), Expect = 1.4e+02, P = 1.0

 Identities = 9/27 (33%), Positives = 17/27 (62%), Frame = +1

Query:  1022 QPVVLVHPVKRYPLRHCRPIITLTLTQ 1048

             Q ++++H + + PL     I+T TLT+

Sbjct:  2665 QRIIIMHVLWQLPLSAKLTIVTATLTK 2745

 Score = 43 (19.5 bits), Expect = 60., Poisson P(2) = 1.0

 Identities = 11/49 (22%), Positives = 21/49 (42%), Frame = +2

Query:   342 KKFGYNGDSLLGQFYKNTGIADAAITTINTVLSAIAAGVGAASAGSLVG 390

             ++FG N D + G   K   + +     +   + A+ +G  A  A +  G

Sbjct:  8795 REFGVNLDKVKGTGRKGRILKEDIQAYVKAAVKALESGAAATGAANGAG 8941

 Score = 42 (19.1 bits), Expect = 2.8, Poisson P(4) = 0.94

 Identities = 9/16 (56%), Positives = 12/16 (75%), Frame = +2

Query:   381 GAASAGSLVGAPIGLL 396

             GAA+ G+  GA +GLL

Sbjct:  8906 GAAATGAANGAGLGLL 8953

 Score = 42 (19.1 bits), Expect = 1.0e+02, Poisson P(2) = 1.0

 Identities = 9/21 (42%), Positives = 14/21 (66%), Frame = +3

Query:   392 PIGLLVSAITSLISGILDASK 412

             P GL+ S I S+I+G+   S+

Sbjct:  4287 PFGLVRSCIFSIITGMRSRSR 4349

 Score = 42 (19.1 bits), Expect = 2.8, Poisson P(4) = 0.94

 Identities = 11/31 (35%), Positives = 15/31 (48%), Frame = +2

Query:   616 AKDDYVFVGSGSTIVNAGDGYDVVDYSKGRT 646

             AK D ++V       N  + YD V  + GRT

Sbjct: 10508 AKQDGIYVSMEGKAGNITNRYDAVLVAIGRT 10600

 Score = 40 (18.2 bits), Expect = 4.4, Poisson P(5) = 0.99

 Identities = 9/36 (25%), Positives = 18/36 (50%), Frame = +2

Query:   296 AGLSTTGPVAGLIASVVSLAISPLSFLGIAKQFDRA 331

             A +    PVAG++  ++  A   +S   +  +F+ A

Sbjct:  8492 ASMEVPAPVAGIVKEIIIKAGDKVSTGTLIMRFETA 8599

>Contig612

          Length = 21,902

  Plus Strand HSPs:

 Score = 55 (25.0 bits), Expect = 5.3, P = 0.99

 Identities = 10/16 (62%), Positives = 13/16 (81%), Frame = +1

Query:   542 TFLNPLLTAGKEERKT 557

             ++L+PL TAGK  RKT

Sbjct:  3541 SYLSPLFTAGKNHRKT 3588

 Score = 49 (22.3 bits), Expect = 3.2, Poisson P(2) = 0.96

 Identities = 11/31 (35%), Positives = 20/31 (64%), Frame = +1

Query:   866 KLSLANINLKDVSFERNGNDLLLKTNNRTAV 896

             +L+LA + L+   F   G+ ++L+ +N TAV

Sbjct:  4840 QLALAELILEQPLFLAYGDKIILRNSNSTAV 4932

>Contig577

          Length = 8894

  Plus Strand HSPs:

 Score = 56 (25.4 bits), Expect = 3.8, P = 0.98

 Identities = 11/26 (42%), Positives = 17/26 (65%), Frame = +3

Query:   394 GLLVSAITSLISGILDASKQAVSEHI 419

             GLL SA+ +++   L  +K A+S HI

Sbjct:  7659 GLLASAVENIVRNALKYTKSAISVHI 7736

>Contig368

          Length = 1932

  Minus Strand HSPs:

 Score = 55 (25.0 bits), Expect = 5.0, P = 0.99

 Identities = 15/57 (26%), Positives = 26/57 (45%), Frame = -3

Query:   555 RKTRQSGKYEFITELKVKGRTDWKVKGVPNSNGVYDFSNLIQHAVTRDNKVLEARLI 611

             RK +Q+G    I   + +    W+VK +    G+  F  +   +VT  N+V+    I

Sbjct:   802 RKLKQNGIIPAIASGRSRTMFPWQVKQLVEQEGMALFVTMNGQSVTHKNQVIAKHTI 632

Parameters:

  E = 5.0, S = 56 (25.4 bits),  E2 = 0.12, S2 = 36

  W = 3, T = 13 (5.9 bits), X = 23 (10.4 bits)

  M = BLOSUM62

  C = 0 (Standard genetic code)

  H = 0, V = 500, B = 250

  -gapdecayrate 0.5 (the default)

Statistics:

  Lambda = 0.315, Lambda/ln2 = 0.454, K = 0.135, H = 0.544 bits/position

  Expected/Observed high score = 58 (26.4 bits) / 4713 (2141.2 bits)

  # of letters in query:  1050 aa

  # of neighborhood words in query:  6103

  # of exact words scoring below T:  67

  Database:  /act/abi/actphrap/auto_act

  # of letters in database:  1,971,947 bp

  # of word hits against database:  1,937,159

  # of failed hit extensions:  1,722,814

  # of successful extensions:  2962

  # of overlapping HSPs discarded:  2941

  # of HSPs reportable:  21

  # of sequences in database:  396

  # of db sequences with at least one HSP:  8

No. of states in DFA:  542 (53 KB)

Total size of DFA:  118 KB (128 KB)

Time to generate neighborhood:  0.01u 0.00s 0.01t  Real: 00:00:00

No. of processors used:  1

Time to search database:  5.81u 0.05s 5.86t  Real: 00:00:12

Total cpu time:  5.90u 0.06s 5.96t  Real: 00:00:12