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OU Aspergillus nidulans cDNA Sequence Blast Server Results

TBLASTN 1.3.9 [29-Oct-93]

Reference:  Altschul, Stephen F., Warren Gish, Webb Miller, Eugene W. Myers,

and David J. Lipman (1990).  Basic local alignment search tool.  J. Mol. Biol.

215:403-410.

Notice:  statistical significance is estimated under the assumption that the

equivalent of one complete reading frame of the database codes for protein and

that significant alignments will involve only coding reading frames.

Query=  GI|3914021|SP|O43123|MAII_EMENI MALEYLACETOACETATE ISOMERASE (MAAI)

        (230 letters)

Database:  /hd1/apache/htdocs/blast_db/asper_est

           12,485 sequences; 4,268,514 total letters.

Searching..................................................done

                                                                     Smallest

                                                                     Poisson

                                                     Reading  High  Probability

Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N

l5g04a1.f1                                                -3   145  4.9e-15   1

v3f09a1.r1                                                +2    73  0.0031    1

z4h02a1.r1                                                -2    73  0.0035    1

m6f12a1.r1                                                -3    60  0.21      1

r5e04a1.r1                                                -2    46  0.41      2

o6e10a1.r1                                                +1    42  0.43      2

g8d05a1.r1                                                +3    43  0.67      2

z4h02a1.f1                                                +1    55  0.72      1

j9f07a1.r1                                                +2    53  0.89      1

d1f08a1.f1                                                +2    38  0.95      2

w4g03a1.r1                                                +1    52  0.97      1

k0a05a1.r1                                                +3    41  0.98      2

f0h12a1.r1                                                +3    41  0.99      2

o5h11a1.r1                                                +1    50  0.99      2

>l5g04a1.f1  

            Length = 268

  Minus Strand HSPs:

 Score = 145 (66.3 bits), Expect = 4.9e-15, P = 4.9e-15

 Identities = 26/26 (100%), Positives = 26/26 (100%), Frame = -3

Query:   205 EALEKENAVKEGHWRTQQDTPTEFRC 230

             EALEKENAVKEGHWRTQQDTPTEFRC

Sbjct:   266 EALEKENAVKEGHWRTQQDTPTEFRC 189

>v3f09a1.r1  

            Length = 422

  Plus Strand HSPs:

 Score = 73 (33.4 bits), Expect = 0.0031, P = 0.0031

 Identities = 19/55 (34%), Positives = 30/55 (54%), Frame = +2

Query:   162 DSAGVFSVGDTITMADVCLIPAVWGAERAGVNLGQYPTIKRVAEALEKENAVKEG 216

             +S   + VGD IT+AD+     V  A  AGV++ ++P +K   E L     V++G

Sbjct:    35 NSKSGYLVGDHITIADISHWGWVAAAGWAGVDIDEFPHLKAWEERLAAREGVEKG 199

>z4h02a1.r1  

            Length = 502

  Minus Strand HSPs:

 Score = 73 (33.4 bits), Expect = 0.0035, P = 0.0035

 Identities = 19/55 (34%), Positives = 30/55 (54%), Frame = -2

Query:   162 DSAGVFSVGDTITMADVCLIPAVWGAERAGVNLGQYPTIKRVAEALEKENAVKEG 216

             +S   + VGD IT+AD+     V  A  AGV++ ++P +K   E L     V++G

Sbjct:   387 NSKSGYLVGDHITIADISHWGWVAAAGWAGVDIDEFPHLKAWEERLAAREGVEKG 223

>m6f12a1.r1  

            Length = 450

  Minus Strand HSPs:

 Score = 60 (27.4 bits), Expect = 0.24, P = 0.21

 Identities = 11/28 (39%), Positives = 17/28 (60%), Frame = -3

Query:    91 FPDNPNPLLPPISNPQQRALVRSLASII 118

             FP +P  +L P+  PQ R   R+L S++

Sbjct:   214 FPSSPGIILTPLIAPQSRKNCRTLCSVV 131

>r5e04a1.r1  

            Length = 515

  Minus Strand HSPs:

 Score = 46 (21.0 bits), Expect = 26., P = 1.0

 Identities = 11/20 (55%), Positives = 13/20 (65%), Frame = -2

Query:    40 NLLKGEQSSTKNTAVNPSAT 59

             +LL  +QSST  T V PS T

Sbjct:   340 SLLPRQQSSTCKTLVRPSRT 281

 Score = 41 (18.8 bits), Expect = 0.52, Poisson P(2) = 0.41

 Identities = 11/29 (37%), Positives = 16/29 (55%), Frame = -1

Query:    45 EQSSTKNTAVNPSATVPTLIIEHVDRSQS 73

             E SS+    V P A+ PT+ I ++D   S

Sbjct:   230 EHSSSVVLPVKPHASEPTMGIWNMDAKAS 144

>o6e10a1.r1  

            Length = 538

  Plus Strand HSPs:

 Score = 42 (19.2 bits), Expect = 96., P = 1.0

 Identities = 8/16 (50%), Positives = 10/16 (62%), Frame = +1

Query:   203 VAEALEKENAVKEGHW 218

             V + LEK +  K GHW

Sbjct:   475 VQDVLEKSDEWK*GHW 522

 Score = 41 (18.8 bits), Expect = 0.55, Poisson P(2) = 0.43

 Identities = 8/16 (50%), Positives = 11/16 (68%), Frame = +2

Query:    43 KGEQSSTKNTAVNPSA 58

             KGE S  K +A+N +A

Sbjct:   419 KGEASGDKQSAINQAA 466

>g8d05a1.r1  

            Length = 366

  Plus Strand HSPs:

 Score = 43 (19.7 bits), Expect = 61., P = 1.0

 Identities = 8/26 (30%), Positives = 15/26 (57%), Frame = +3

Query:    94 NPNPLLPPISNPQQRALVRSLASIIA 119

             +P P +PP ++P    L  S  S+++

Sbjct:   222 SPFPRVPPTASPPTWVLTSSSTSVLS 299

 Score = 39 (17.8 bits), Expect = 1.1, Poisson P(2) = 0.67

 Identities = 13/42 (30%), Positives = 16/42 (38%), Frame = +3

Query:    60 VPTLIIEHVDRSQSPITITQSLAALEYLDEAFPDNPNPLLPP 101

             VPT  +  V R    +  T S+ A       FP  P    PP

Sbjct:   135 VPTRPVVSVTRLSRDMLSTVSVCAAVAARSPFPRVPPTASPP 260

>z4h02a1.f1  

            Length = 453

  Plus Strand HSPs:

 Score = 55 (25.2 bits), Expect = 1.3, P = 0.72

 Identities = 14/37 (37%), Positives = 22/37 (59%), Frame = +1

Query:   162 DSAGVFSVGDTITMADVCLIPAVWGAERAGVNLGQYP 198

             +S   + VGD IT+AD+     V  A  AGV++ ++P

Sbjct:   337 NSKSGYLVGDHITIADISHWGWVAAAGWAGVDIDEFP 447

>j9f07a1.r1  

            Length = 379

  Plus Strand HSPs:

 Score = 53 (24.2 bits), Expect = 2.2, P = 0.89

 Identities = 12/29 (41%), Positives = 15/29 (51%), Frame = +2

Query:    97 PLLPPISNPQQRALVRSLASIIACDIQPV 125

             P  P  S  +QR L RS   +  CDI P+

Sbjct:   113 PTQPEQSTSRQRGLSRSSRPVDECDICPI 199

>d1f08a1.f1  

            Length = 309

  Plus Strand HSPs:

 Score = 38 (17.4 bits), Expect = 3.0e+02, P = 1.0

 Identities = 5/16 (31%), Positives = 10/16 (62%), Frame = +2

Query:   172 TITMADVCLIPAVWGA 187

             TI +  + ++P  WG+

Sbjct:   173 TINLHSIFILPLTWGS 220

 Score = 37 (16.9 bits), Expect = 3.1, Poisson P(2) = 0.95

 Identities = 10/34 (29%), Positives = 15/34 (44%), Frame = +3

Query:   102 ISNPQQRALVRSLASIIACDIQPVTNLRILQRVA 135

             + NP   +L     S I C + P   LR+   +A

Sbjct:    90 LHNPATVSLTFRSCSRICCSVWPSALLRLSISIA 191

>w4g03a1.r1  

            Length = 458

  Plus Strand HSPs:

 Score = 52 (23.8 bits), Expect = 3.4, P = 0.97

 Identities = 12/31 (38%), Positives = 18/31 (58%), Frame = +1

Query:    88 DEAFPDNPNPLLPPISNPQQRALVRSLASII 118

             D +  D+ +PLL P  +PQ +A  R   +II

Sbjct:   292 DPSARDDTSPLLEPNRDPQVKAQARDTDAII 384

>k0a05a1.r1  

            Length = 349

  Plus Strand HSPs:

 Score = 41 (18.8 bits), Expect = 1.2e+02, P = 1.0

 Identities = 9/28 (32%), Positives = 16/28 (57%), Frame = +3

Query:    32 ISYTSVPINLLKGEQSSTKNTAVNPSAT 59

             ++ TSVP+NL     S+   ++  P +T

Sbjct:   159 LTSTSVPVNLPPKNSSALSPSSRTPPST 242

 Score = 37 (16.9 bits), Expect = 3.8, Poisson P(2) = 0.98

 Identities = 8/21 (38%), Positives = 11/21 (52%), Frame = +1

Query:    92 PDNPNPLLPPISNPQQRALVR 112

             P  P+P+  P   P+Q A  R

Sbjct:   220 PPEPHPVQDPHLVPEQTARYR 282

>f0h12a1.r1  

            Length = 385

  Plus Strand HSPs:

 Score = 41 (18.8 bits), Expect = 1.2e+02, P = 1.0

 Identities = 9/28 (32%), Positives = 16/28 (57%), Frame = +3

Query:    32 ISYTSVPINLLKGEQSSTKNTAVNPSAT 59

             ++ TSVP+NL     S+   ++  P +T

Sbjct:   237 LTSTSVPVNLPPKNSSALSPSSRTPPST 320

 Score = 37 (16.9 bits), Expect = 4.5, Poisson P(2) = 0.99

 Identities = 8/21 (38%), Positives = 11/21 (52%), Frame = +1

Query:    92 PDNPNPLLPPISNPQQRALVR 112

             P  P+P+  P   P+Q A  R

Sbjct:   298 PPEPHPVQDPHLVPEQTARYR 360

>o5h11a1.r1  

            Length = 391

  Plus Strand HSPs:

 Score = 50 (22.9 bits), Expect = 6.2, P = 1.0

 Identities = 11/36 (30%), Positives = 18/36 (50%), Frame = +1

Query:    66 EHVDRSQSPITITQSLAALEYLDEAFPDNPNPLLPP 101

             +H+DR   P   +   ++L  L +  P  P+P  PP

Sbjct:   181 QHLDRHFYPEYPSAFFSSLHSLPQPLPPPPSPRSPP 288

 Score = 37 (16.9 bits), Expect = 4.6, Poisson P(2) = 0.99

 Identities = 8/18 (44%), Positives = 10/18 (55%), Frame = +3

Query:   121 DIQPVTNLRILQRVAPFG 138

             D+QP +    L RV P G

Sbjct:   333 DLQPRSTKPFLLRVMPLG 386

Parameters:

  E = 5.0, S = 52 (23.8 bits),  E2 = 0.15, S2 = 35

  W = 3, T = 13 (5.9 bits), X = 22 (10.1 bits)

  M = BLOSUM62

  C = 0 (Standard genetic code)

  H = 0, V = 500, B = 250

  -gapdecayrate 0.5 (the default)

Statistics:

  Lambda = 0.317, Lambda/ln2 = 0.457, K = 0.131, H = 0.540 bits/position

  Expected/Observed high score = 54 (24.7 bits) / 145 (66.3 bits)

  # of letters in query:  230 aa

  # of neighborhood words in query:  1496

  # of exact words scoring below T:  15

  Database:  /hd1/apache/htdocs/blast_db/asper_est

  # of letters in database:  4,268,514 bp

  # of word hits against database:  1,092,684

  # of failed hit extensions:  930,532

  # of successful extensions:  1990

  # of overlapping HSPs discarded:  1969

  # of HSPs reportable:  21

  # of sequences in database:  12,485

  # of db sequences with at least one HSP:  14

No. of states in DFA:  476 (47 KB)

Total size of DFA:  64 KB (64 KB)

Time to generate neighborhood:  0.00u 0.00s 0.00t  Real: 00:00:00

No. of processors used:  1

Time to search database:  3.97u 0.06s 4.03t  Real: 00:00:08

Total cpu time:  4.04u 0.10s 4.14t  Real: 00:00:08