The Sanger Centre blast servers are
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TBLASTN 2.0a13MP-WashU [10-Jun-1997] [Build 23:08:22 Jun 10 1997]
Reference: Gish, Warren (1994-1997). unpublished.
Altschul, Stephen F., Warren Gish, Webb Miller, Eugene W. Myers, and David J.
Lipman (1990). Basic local alignment search tool. J. Mol. Biol. 215:403-10.
Notice: statistical significance is estimated under the assumption that the
equivalent of one complete reading frame of the database codes for protein and
that significant alignments will involve only coding reading frames.
Query= query_seq
(399 letters)
Database: ML_Sanger.dbs
32 sequences; 1,197,952 total letters.
Searching....10....20....30....40....50....60....70....80....90....100% done
Smallest
Sum
Reading High Probability
Sequences producing High-scoring Segment Pairs: Frame Score P(N) N
MLCB637 +2 1481 1.4e-152 1
>MLCB637 [Full Sequence]
Length = 44,882
Plus Strand HSPs:
Score = 1481 (521.3 bits), Expect = 1.4e-152, P = 1.4e-152
Identities = 298/336 (88%), Positives = 298/336 (88%), Frame = +2
Query: 64 MKLAIIGGDGIGPEVVAQAVKILDVVLPGVQKTTYDLGARRYHTTGELLPESVLAELREH 123
MKLAIIGGDGIGPEVVAQAVKILDVVLPGVQKTTYDLGARRYHTTGELLPESVLAELREH
Sbjct: 29798 MKLAIIGGDGIGPEVVAQAVKILDVVLPGVQKTTYDLGARRYHTTGELLPESVLAELREH 29977
Query: 124 DAILLGAVGDPSVPSGVXXXXXXXXXXXXXDHHINLRPARLYPGVNSLLAGKPDIDFVVV 183
DAILLGAVGDPSVPSGV DHHINLRPARLYPGVNSLLAGKPDIDFVVV
Sbjct: 29978 DAILLGAVGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVNSLLAGKPDIDFVVV 30157
Query: 184 RXXXXXXXXXXXXAIRVGTPNEVATEVSVNTAFGVRRVVQDAFERARQRRKHLTLVHKNN 243
R AIRVGTPNEVATEVSVNTAFGVRRVVQDAFERARQRRKHLTLVHKNN
Sbjct: 30158 REGTEGPYTGTGGAIRVGTPNEVATEVSVNTAFGVRRVVQDAFERARQRRKHLTLVHKNN 30337
Query: 244 VLTYAGTLWCRIVQEVGEKYPDVEVVYQHIDAATIYLVTEPSRFDVIVTDNLFGDIITDX 303
VLTYAGTLWCRIVQEVGEKYPDVEVVYQHIDAATIYLVTEPSRFDVIVTDNLFGDIITD
Sbjct: 30338 VLTYAGTLWCRIVQEVGEKYPDVEVVYQHIDAATIYLVTEPSRFDVIVTDNLFGDIITDL 30517
Query: 304 XXXXXXXXXXXXSGNIDATRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSLALLLAHLGE 363
SGNIDATRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSLALLLAHLGE
Sbjct: 30518 AAAVCGGIALAASGNIDATRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSLALLLAHLGE 30697
Query: 364 DEPAARLDQAVASYLATRGNGRFSTGEVGERIAAAL 399
DEPAARLDQAVASYLATRGNGRFSTGEVGERIAAAL
Sbjct: 30698 DEPAARLDQAVASYLATRGNGRFSTGEVGERIAAAL 30805
Parameters:
P=6
B=50
filter=seg
ctxfactor=6.00
E=10
Query ----- As Used ----- ----- Computed ----
Frame MatID Matrix name Lambda K H Lambda K H
+0 0 BLOSUM62 0.318 0.137 0.395 same same same
q=9 r=2 0.244 0.0300 0.180
Query
Frame MatID Length Eff.Length E S W T X E2 S2
+0 0 399 361 10. 56 3 13 22 0.057 38
34 0.063 42
Statistics:
Database: ML_Sanger.dbs
Title: ML_Sanger.dbs
Release date: unknown
Posted date: 6:18 PM BST May 4, 1999
Format: BLAST
# of letters in database: 1,197,952
# of sequences in database: 32
# of database sequences satisfying E: 1
No. of states in DFA: 479 (102 KB)
Total size of DFA: 150 KB (192 KB)
Time to generate neighborhood: 0.00u 0.00s 0.00t Elapsed: 00:00:00
No. of threads or processors used: 6
Search cpu time: 0.68u 0.10s 0.78t Elapsed: 00:00:00
Total cpu time: 0.73u 0.16s 0.90t Elapsed: 00:00:00
Start: Tue Oct 12 23:21:33 1999 End: Tue Oct 12 23:21:33 1999
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